Recombinant:Article Title: Netrin1 patterns the dorsal spinal cord through modulation of Bmp signaling.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti neurofilament Cell Signaling Technology Cat#C28E10; RRID: AB_10828120 Mouse anti tubulin b3 (Tubb3)/Tuj1 BioLegend Cat#801202; RRID:AB_10063408 Rabbit anti laminin Abcam Cat#11575; RRID: AB_298179 Mouse anti chicken transitin/nestin Developmental Studies Hybridoma bank Cat#EAP3; RRID: AB_2282449 Mouse anti myc tag Abcam Cat#ab32; RRID: AB_303599 Mouse anti GFP Invitrogen Cat#A-11120 Goat anti mouse netrin1 R&D Systems Cat#AF1109; RRID: AB_2298775 Goat anti chicken netrin1 R&D Systems Cat#AF128; RRID:AB_354716 Goat anti chicken netrin2 R&D Systems Cat#AF127; RR1D: AB_2154709 Goat anti Sox2 Santa Cruz Biotechnology Cat# sc-17320; RRID:AB_2286684 Mouse anti P27 BD Biosciences Cat#610241; RRID:AB_397636 Rabbit anti cleaved caspase 3 BD Biosciences Cat#559565; RRID:AB_397274 Rabbit anti phospho histone H3 Cell signaling Technology Cat#9701; RRID:AB_33153 Goat anti Lhx2 Santa Cruz Biotechnology Cat#Sc-19344; RRID:AB_2135660 Rabbit anti Lhx2 Gift from Tom Jessell; Liem et al.13 N/A Mouse anti Lhx1/5 Developmental Studies Hybridoma bank Cat#4F2; RRID:AB_531784 Guinea Pig anti Tlx3 Gift from Thomas Muller; Muller et al.76 N/A Mouse anti Isl1/2 Developmental Studies Hybridoma bank Cat#39.4DS Goat anti Isl1/2 R&D Systems CatAF1837, RRID:AB_2126324 Rabbit anti Pax2 Invitrogen Cat#71-6000 Guinea Pig anti Olig2 Gift from Bennett Novitch; Novitch et al.62 N/A Rabbit anti Math1/Atoh1 Helms and Johnson77 N/A Goat anti Ascl1/Mash1 R&D Systems Cat#AF2567; RRID:AB_2059505 Guinea Pig anti Foxd3 sera Gift from Thomas Muller; Muller et al.76 N/A Rabbit anti Gapdh Protientech Cat# 10494-1-AP; RRID:AB_2263076 Rabbit anti Phospho-Smad1 (Ser463/465)/Smad5 (Ser463/465)/Smad8 (Ser465/467) (D5B10) Cell Signaling Technology Cat#11971; RRID:AB_2797785 Chemicals, peptides, and recombinant proteins Mouse Recombinant netrin1 R&D Ca#1109-N1-025 Human Recombinant Bmp4 Thermo Fisher Scientific Cat#PHC9534 Retinoic acid Sigma Aldrich Cat#R2625 Human basic FGF (bFGF) Thermo Fisher Scientific Cat#PHG0023 Neurobasal medium Thermo Fisher Scientific Cat#21103049 B27 supplement Fisher Scientific Cat#17-504-044 N2 supplement Thermo Fisher Scientific Cat#17502048 CHIR99021 Tocris Cat#4423 (Continued on next page) 18 Cell Reports --, 114954, --, 2024 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Paraformaldehyde 16% Sigma-Aldrich Cat#28906 Prolong Gold Thermo Fisher Scientific Cat#P36930 Blotting-Grade Blocker Bio Rad Cat#1706404 cOmpleteTM, EDTA-free Protease Inhibitor Cocktail Roche Cat#04693132001 PhosSTOPTM, Phosphatase Inhibitor Roche Cat#4906845001 30% Acrylamide/Bis Solution, 37.5:1 Bio Rad Cat#1610158 10x Tris/Glycine/SDS Bio Rad Cat#1610732 Critical commercial assays SuperSignalTM West Femto Maximum Sensitivity Substrate Thermo Scientific Cat#34094 RNeasy plus mini kit Qiagen Cat#74106 DIG RNA Labeling Kit Roche, Millipore Cat#11175025910 Superscript IV First-Strand synthesis kit Thermo Fisher Scientific Cat#18091050 Deposited data Bulk RNA-Seq Gene Expression Omnibus (GEO) repository GEO: GSE246903 Experimental models: Cell lines Cos7 cells ATTC ATCC CRL-1651 MM13 mouse embryonic stem cell line Gift from Tom Jessell, Columbia University Wichterle et al.54 N/A Experimental models: Organisms/strains Mouse: Netrin1 null line Lisa Goodrich Lab, Harvard University Yung et al.60 N/A Chicken: Fertile Leghorn chicken eggs CJ Eggs, Sylmar, Ca N/A Oligonucleotides Forward in situ probe for chicken netrin1: 50-GACATCCACATCCTGAAAGCGGA-30 this paper N/A Reverse in situ reverse probe for chicken netrin1with T7 sequence attached: 50- GACTAATACGA CTCACTATAGGGTTTCCCCTTCCATCCCTCAA-30 this paper N/A Forward in situ probe for chicken netrin2: 50- GACTTTCTTGTGCAGCAGAGACG-30 this paper N/A Reverse in situ probe for chicken netrin2 with T3 sequence attached: 50-GACATTAACCC TCACTAAAGGGACTCTCC TCTCTTCCTGCCAC this paper N/A Forward in situ probe for mouse Id1: 50- TCAGGAGGCAAGAAGAAAAA-30 this paper N/A Reverse in situ probe for mouse Id1 with T3 sequence attached: 50- GAGATTAACCCTC ACTAAAGGGAAGAAATCCGAGAAGCACGAA-30 this paper N/A Forward in situ probe for mouse Id3: 50- GACTCTGGGACCCTCTCTCC-30 this paper N/A Reverse in situ probe for mouse Id3 with T3 sequence attached: 50-GAGATTAACCCT CACTAAAGGGATAATCAGGGCAGCAGAGCTT- 30 this paper N/A Forward qPCR primer for Lhx2: 50- CAGCTTGCGCAAAAGACC-30 this paper N/A Reverse qPCR primer for Lhx2: 50- TAAAAGGTTGCGCCTGAACT-30 this paper N/A (Continued on next page) Cell Reports --, 114954, --, 2024 19 OPEN ACCESS
In Situ:Article Title: Netrin1 in spinal cord development: More than an axon guidance molecule
Article Snippet: .. 50 Mouse anti LHX1/5 Developmental Studies Hybridoma bank Cat#4F2;RRID:AB _531784 Guinea Pig anti TLX3 Gift from Thomas Muller; (Muller et al., 2005 ) N/A Mouse anti ISL1/2 Developmental Studies Hybridoma bank Cat#39.4DS Goat anti ISL1/2 R&D Systems CatAF1837, RRID:AB_212632 4 Rabbit anti Pax2 Invitrogen Cat#71-6000 Guinea Pig anti Olig2 Novitch et al., 2001148 N/A Rabbit anti Math1/Atoh1 Helms & Johnson, 199838 N/A Goat anti ASCL1/MASH1 R&D Systems Cat#AF2567; RRID:AB_205950 5 Guinea Pig anti FOXD3 sera Gift from Thomas Muller; (Muller et al., 2005) N/A Rabbit anti GAPDH Protientech Cat# 10494-1-AP; RRID:AB_226307 6 Rabbit anti Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad9 (Ser465/467) (D5B10) Cell Signaling Technology Cat#11971; RRID:AB_279778 5 Oligonucleotides In situ probe for Chicken netrin1: Forward 5’- GACATCCACATCCTGAAAGCGGA Reverse with T7 sequence attached: GACTAA TACGACTCACTATAGGGTTTCCCCTTC CATCCCTCAA This Paper N/A In situ probe for Chicken netrin2: Forward 5’- GACTTTCTTGTGCAGCAGAGACG-3’ Reverse with T3 sequence attached 5’- GACATTAACCCTCACTAAAGGGACTCT CC TCTCTTCCTGCCAC This Paper N/A In situ probe for Mouse Id1: Forward 5’- TCAGGAGGCAAGAAGAAAAA-3’ Reverse with T3 sequence attached 5’- GAGATTAACCCTCACTAAAGGGAAGA AATCCGAGAAGCACGAA-3’ This Paper N/A 51 In situ probe for Mouse Id3: Forward 5’- GACTCTGGGACCCTCTCTCC-3’ Reverse with T3 sequence attached 5’- GAGATTAACCCTCACTAAAGGGATAAT CAGGGCAGCAGAGCTT- 3’ This Paper N/A qPCR primer for Lhx2: Forward 5’- CAGCTTGCGCAAAAGACC3’ reverse: 5’- TAAAAGGTTGCGCCTGAACT-3’ This Paper N/A qPCR primer for Lhx9: Forward 5’- CAGGCCTGACCAAAAGAGTT-3’ Reverse 5’- TGCCGTCAGCTTTATCAACA-3’ This Paper N/A qPCR primer for FOXD3: Forward 5’- CCCCAACACTGACCAACAG3’ Reverse 5’- GTTTGCTCCGCCAGCTTA-3’ This Paper N/A qPCR primer for ISL1: Forward 5’- AGGACAAGAAACGCAGCATC-3’ Reverse 5’- TTCCTGTCATCCCCTGGATA3’ This Paper N/A qPCR primer for GAPDH: Forward 5’- GGCCTTCCGTGTTCCTAC-3’ Reverse 5’- TGTCATCATACTTGGCAGGTT-3’ This Paper N/A 52 Chapter 3 - Assessing the existence of netrin1 isoforms in the developing spinal cord. ..
Sequencing:Article Title: Netrin1 in spinal cord development: More than an axon guidance molecule
Article Snippet: .. 50 Mouse anti LHX1/5 Developmental Studies Hybridoma bank Cat#4F2;RRID:AB _531784 Guinea Pig anti TLX3 Gift from Thomas Muller; (Muller et al., 2005 ) N/A Mouse anti ISL1/2 Developmental Studies Hybridoma bank Cat#39.4DS Goat anti ISL1/2 R&D Systems CatAF1837, RRID:AB_212632 4 Rabbit anti Pax2 Invitrogen Cat#71-6000 Guinea Pig anti Olig2 Novitch et al., 2001148 N/A Rabbit anti Math1/Atoh1 Helms & Johnson, 199838 N/A Goat anti ASCL1/MASH1 R&D Systems Cat#AF2567; RRID:AB_205950 5 Guinea Pig anti FOXD3 sera Gift from Thomas Muller; (Muller et al., 2005) N/A Rabbit anti GAPDH Protientech Cat# 10494-1-AP; RRID:AB_226307 6 Rabbit anti Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad9 (Ser465/467) (D5B10) Cell Signaling Technology Cat#11971; RRID:AB_279778 5 Oligonucleotides In situ probe for Chicken netrin1: Forward 5’- GACATCCACATCCTGAAAGCGGA Reverse with T7 sequence attached: GACTAA TACGACTCACTATAGGGTTTCCCCTTC CATCCCTCAA This Paper N/A In situ probe for Chicken netrin2: Forward 5’- GACTTTCTTGTGCAGCAGAGACG-3’ Reverse with T3 sequence attached 5’- GACATTAACCCTCACTAAAGGGACTCT CC TCTCTTCCTGCCAC This Paper N/A In situ probe for Mouse Id1: Forward 5’- TCAGGAGGCAAGAAGAAAAA-3’ Reverse with T3 sequence attached 5’- GAGATTAACCCTCACTAAAGGGAAGA AATCCGAGAAGCACGAA-3’ This Paper N/A 51 In situ probe for Mouse Id3: Forward 5’- GACTCTGGGACCCTCTCTCC-3’ Reverse with T3 sequence attached 5’- GAGATTAACCCTCACTAAAGGGATAAT CAGGGCAGCAGAGCTT- 3’ This Paper N/A qPCR primer for Lhx2: Forward 5’- CAGCTTGCGCAAAAGACC3’ reverse: 5’- TAAAAGGTTGCGCCTGAACT-3’ This Paper N/A qPCR primer for Lhx9: Forward 5’- CAGGCCTGACCAAAAGAGTT-3’ Reverse 5’- TGCCGTCAGCTTTATCAACA-3’ This Paper N/A qPCR primer for FOXD3: Forward 5’- CCCCAACACTGACCAACAG3’ Reverse 5’- GTTTGCTCCGCCAGCTTA-3’ This Paper N/A qPCR primer for ISL1: Forward 5’- AGGACAAGAAACGCAGCATC-3’ Reverse 5’- TTCCTGTCATCCCCTGGATA3’ This Paper N/A qPCR primer for GAPDH: Forward 5’- GGCCTTCCGTGTTCCTAC-3’ Reverse 5’- TGTCATCATACTTGGCAGGTT-3’ This Paper N/A 52 Chapter 3 - Assessing the existence of netrin1 isoforms in the developing spinal cord. ..
Real-time Polymerase Chain Reaction:Article Title: Netrin1 in spinal cord development: More than an axon guidance molecule
Article Snippet: .. 50 Mouse anti LHX1/5 Developmental Studies Hybridoma bank Cat#4F2;RRID:AB _531784 Guinea Pig anti TLX3 Gift from Thomas Muller; (Muller et al., 2005 ) N/A Mouse anti ISL1/2 Developmental Studies Hybridoma bank Cat#39.4DS Goat anti ISL1/2 R&D Systems CatAF1837, RRID:AB_212632 4 Rabbit anti Pax2 Invitrogen Cat#71-6000 Guinea Pig anti Olig2 Novitch et al., 2001148 N/A Rabbit anti Math1/Atoh1 Helms & Johnson, 199838 N/A Goat anti ASCL1/MASH1 R&D Systems Cat#AF2567; RRID:AB_205950 5 Guinea Pig anti FOXD3 sera Gift from Thomas Muller; (Muller et al., 2005) N/A Rabbit anti GAPDH Protientech Cat# 10494-1-AP; RRID:AB_226307 6 Rabbit anti Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad9 (Ser465/467) (D5B10) Cell Signaling Technology Cat#11971; RRID:AB_279778 5 Oligonucleotides In situ probe for Chicken netrin1: Forward 5’- GACATCCACATCCTGAAAGCGGA Reverse with T7 sequence attached: GACTAA TACGACTCACTATAGGGTTTCCCCTTC CATCCCTCAA This Paper N/A In situ probe for Chicken netrin2: Forward 5’- GACTTTCTTGTGCAGCAGAGACG-3’ Reverse with T3 sequence attached 5’- GACATTAACCCTCACTAAAGGGACTCT CC TCTCTTCCTGCCAC This Paper N/A In situ probe for Mouse Id1: Forward 5’- TCAGGAGGCAAGAAGAAAAA-3’ Reverse with T3 sequence attached 5’- GAGATTAACCCTCACTAAAGGGAAGA AATCCGAGAAGCACGAA-3’ This Paper N/A 51 In situ probe for Mouse Id3: Forward 5’- GACTCTGGGACCCTCTCTCC-3’ Reverse with T3 sequence attached 5’- GAGATTAACCCTCACTAAAGGGATAAT CAGGGCAGCAGAGCTT- 3’ This Paper N/A qPCR primer for Lhx2: Forward 5’- CAGCTTGCGCAAAAGACC3’ reverse: 5’- TAAAAGGTTGCGCCTGAACT-3’ This Paper N/A qPCR primer for Lhx9: Forward 5’- CAGGCCTGACCAAAAGAGTT-3’ Reverse 5’- TGCCGTCAGCTTTATCAACA-3’ This Paper N/A qPCR primer for FOXD3: Forward 5’- CCCCAACACTGACCAACAG3’ Reverse 5’- GTTTGCTCCGCCAGCTTA-3’ This Paper N/A qPCR primer for ISL1: Forward 5’- AGGACAAGAAACGCAGCATC-3’ Reverse 5’- TTCCTGTCATCCCCTGGATA3’ This Paper N/A qPCR primer for GAPDH: Forward 5’- GGCCTTCCGTGTTCCTAC-3’ Reverse 5’- TGTCATCATACTTGGCAGGTT-3’ This Paper N/A 52 Chapter 3 - Assessing the existence of netrin1 isoforms in the developing spinal cord. ..
|
R&D Systems is a verified supplier 
R&D Systems manufactures this product 